p66Shc protein has been proposed to be an indispensable factor for p53-dependent, mitochondria-mediated apoptosis in mice. Here, we show that p66Shc plays a pro-apoptotic role also in cell lines of human origin such as SaOs-2 and HeLa, where p53 is either absent or inactivated, thus, suggesting that p66Shc pro-apoptotic role is independent from the presence of a functional form of p53. The active form of p66Shc is phosphorylated in Serine 36. We confirm the importance of Serine 36 phosphorylation for p66Shc pro-apoptotic role, and our results suggest that the kinase involved in this process is activated independently from p53.
p66Shc gene has a pro-apoptotic role in human cell lines and it is activated by a p53-independent pathway / Tiberi, Luca; Faisal, Amir; Rossi, Matteo; Tella, Lucia Di; Franceschi, Claudio; Salvioli, Stefano. - In: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. - ISSN 0006-291X. - ELETTRONICO. - 342:2(2006), pp. 503-508. [10.1016/j.bbrc.2006.02.007]
p66Shc gene has a pro-apoptotic role in human cell lines and it is activated by a p53-independent pathway
Tiberi, Luca;
2006-01-01
Abstract
p66Shc protein has been proposed to be an indispensable factor for p53-dependent, mitochondria-mediated apoptosis in mice. Here, we show that p66Shc plays a pro-apoptotic role also in cell lines of human origin such as SaOs-2 and HeLa, where p53 is either absent or inactivated, thus, suggesting that p66Shc pro-apoptotic role is independent from the presence of a functional form of p53. The active form of p66Shc is phosphorylated in Serine 36. We confirm the importance of Serine 36 phosphorylation for p66Shc pro-apoptotic role, and our results suggest that the kinase involved in this process is activated independently from p53.File | Dimensione | Formato | |
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