A PDMS chip is fabricated using soft lithography and applied to investigate the formation and division of nitrobenzene (NB) droplets in a two-phase system stabilized by oleic acid. Using an integrated on-chip flow cytometer setup, effected with optical fibers, droplet size distributions are analyzed in situ based on optical signal intensities. By controlling the hydrodynamic flow focusing, uniform droplets of sizes between 100 μm and 300 μm are created with precise size control. Cross-flow shearing allows one to divide these droplets into anything from 2 to 9 individual droplets, depending on flow parameters.

Uniform droplet splitting and detection using Lab-on-Chip flow cytometry on a microfluidic PDMS device / Kunstmann Olsen, Casper; Hanczyc, Martin Michael; Hoyland, James; Rasmussen, Steen; Rubahn, Horst Günter. - In: SENSORS AND ACTUATORS. B, CHEMICAL. - ISSN 0925-4005. - 229:(2016), pp. 7-13. [10.1016/j.snb.2016.01.120]

Uniform droplet splitting and detection using Lab-on-Chip flow cytometry on a microfluidic PDMS device

Hanczyc, Martin Michael;
2016-01-01

Abstract

A PDMS chip is fabricated using soft lithography and applied to investigate the formation and division of nitrobenzene (NB) droplets in a two-phase system stabilized by oleic acid. Using an integrated on-chip flow cytometer setup, effected with optical fibers, droplet size distributions are analyzed in situ based on optical signal intensities. By controlling the hydrodynamic flow focusing, uniform droplets of sizes between 100 μm and 300 μm are created with precise size control. Cross-flow shearing allows one to divide these droplets into anything from 2 to 9 individual droplets, depending on flow parameters.
2016
Kunstmann Olsen, Casper; Hanczyc, Martin Michael; Hoyland, James; Rasmussen, Steen; Rubahn, Horst Günter
Uniform droplet splitting and detection using Lab-on-Chip flow cytometry on a microfluidic PDMS device / Kunstmann Olsen, Casper; Hanczyc, Martin Michael; Hoyland, James; Rasmussen, Steen; Rubahn, Horst Günter. - In: SENSORS AND ACTUATORS. B, CHEMICAL. - ISSN 0925-4005. - 229:(2016), pp. 7-13. [10.1016/j.snb.2016.01.120]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11572/138845
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