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EnglishThe ERG gene is fused to TMPRSS2 in approximately 50% of prostate cancers (PrCa), resulting in its overexpression. However, whether this is the sole mechanism underlying ERG elevation in PrCa is currently unclear. Here we report that ERG ubiquitination and degradation are governed by the Cullin 3-based ubiquitin ligase SPOP and that deficiency in this pathway leads to aberrant elevation of the ERG oncoprotein. Specifically, we find that truncated ERG (DERG), encoded by the ERG fusion gene, is stabilized by evading SPOP-mediated destruction, whereas prostate cancer-associated SPOP mutants are also deficient in promoting ERG ubiquitination. Furthermore, we show that the SPOP/ERG interaction is modulated by CKI-mediated phosphorylation. Importantly, we demonstrate that DNA damage drugs, topoisomerase inhibitors, can trigger CKI activation to restore the SPOP/DERG interaction and its consequent degradation. Therefore, SPOP functions as a tumor suppressor to negatively regulate the stability of the ERG oncoprotein in prostate cancer.
SPOP Promotes Ubiquitination and Degradation of the ERG Oncoprotein to Suppress Prostate Cancer Progression / Gan, Wenjian; Dai, Xiangpeng; Lunardi, Andrea; Li, Zhen; Inuzuka, Hiroyuki; Liu, Pengda; Varmeh, Shoreh; Zhang, Jinfang; Cheng, Liang; Sun, Yin; Asara, John M.; Beck, Andrew H.; Huang, Jiaoti; Pandolfi, Pier Paolo; Wei, Wenyi. - In: MOLECULAR CELL. - ISSN 1097-2765. - 59:6(2015), pp. 917-930. [10.1016/j.molcel.2015.07.026]
| Titolo: | SPOP Promotes Ubiquitination and Degradation of the ERG Oncoprotein to Suppress Prostate Cancer Progression | |
| Autori: | Gan, Wenjian; Dai, Xiangpeng; Lunardi, Andrea; Li, Zhen; Inuzuka, Hiroyuki; Liu, Pengda; Varmeh, Shoreh; Zhang, Jinfang; Cheng, Liang; Sun, Yin; Asara, John M.; Beck, Andrew H.; Huang, Jiaoti; Pandolfi, Pier Paolo; Wei, Wenyi | |
| Autori Unitn: | ||
| Titolo del periodico: | MOLECULAR CELL | |
| Anno di pubblicazione: | 2015 | |
| Numero e parte del fascicolo: | 6 | |
| Codice identificativo Scopus: | 2-s2.0-84941752941 | |
| Codice identificativo Pubmed: | 26344095 | |
| Codice identificativo WOS: | WOS:000365166500006 | |
| Digital Object Identifier (DOI): | http://dx.doi.org/10.1016/j.molcel.2015.07.026 | |
| Handle: | http://hdl.handle.net/11572/128146 | |
| Citazione: | SPOP Promotes Ubiquitination and Degradation of the ERG Oncoprotein to Suppress Prostate Cancer Progression / Gan, Wenjian; Dai, Xiangpeng; Lunardi, Andrea; Li, Zhen; Inuzuka, Hiroyuki; Liu, Pengda; Varmeh, Shoreh; Zhang, Jinfang; Cheng, Liang; Sun, Yin; Asara, John M.; Beck, Andrew H.; Huang, Jiaoti; Pandolfi, Pier Paolo; Wei, Wenyi. - In: MOLECULAR CELL. - ISSN 1097-2765. - 59:6(2015), pp. 917-930. [10.1016/j.molcel.2015.07.026] | |
| Appare nelle tipologie: | 03.1 Articolo su rivista (Journal article) |
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| File | Descrizione | Tipologia | Licenza | |
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| PIIS1097276515005845.pdf | Versione editoriale (Publisher’s layout) | Tutti i diritti riservati (All rights reserved) | Open Access Visualizza/Apri | |
| nihms720821.pdf | Post-print referato (Refereed author’s manuscript) |  | Open Access Visualizza/Apri |
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