An in vitro selection method for ligand-responsive RNA sensors was developed that exploited strand displacement reactions. The RNA library was based on the thiamine pyrophosphate (TPP) riboswitch, and RNA sequences capable of hybridizing to a target duplex DNA in a TPP regulated manner were identified. After three rounds of selection, RNA molecules that mediated a strand exchange reaction upon TPP binding were enriched. The enriched sequences also showed riboswitch activity. Our results demonstrated that small-molecule-responsive nucleic acid sensors can be selected to control the activity of target nucleic acid circuitry.

In vitro selection for small molecule triggered strand displacement and riboswitch activity / Martini, Laura; A. J., Meyer; J. W., Ellefson; J. N., Milligan; Forlin, Michele; A. D., Ellington; Mansy, Sheref Samir. - In: ACS SYNTHETIC BIOLOGY. - ISSN 2161-5063. - ELETTRONICO. - 4:10(2015), pp. 1144-1150. [10.1021/acssynbio.5b00054]

In vitro selection for small molecule triggered strand displacement and riboswitch activity

Martini, Laura;Forlin, Michele;Mansy, Sheref Samir
2015-01-01

Abstract

An in vitro selection method for ligand-responsive RNA sensors was developed that exploited strand displacement reactions. The RNA library was based on the thiamine pyrophosphate (TPP) riboswitch, and RNA sequences capable of hybridizing to a target duplex DNA in a TPP regulated manner were identified. After three rounds of selection, RNA molecules that mediated a strand exchange reaction upon TPP binding were enriched. The enriched sequences also showed riboswitch activity. Our results demonstrated that small-molecule-responsive nucleic acid sensors can be selected to control the activity of target nucleic acid circuitry.
2015
10
Martini, Laura; A. J., Meyer; J. W., Ellefson; J. N., Milligan; Forlin, Michele; A. D., Ellington; Mansy, Sheref Samir
In vitro selection for small molecule triggered strand displacement and riboswitch activity / Martini, Laura; A. J., Meyer; J. W., Ellefson; J. N., Milligan; Forlin, Michele; A. D., Ellington; Mansy, Sheref Samir. - In: ACS SYNTHETIC BIOLOGY. - ISSN 2161-5063. - ELETTRONICO. - 4:10(2015), pp. 1144-1150. [10.1021/acssynbio.5b00054]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11572/127439
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