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EnglishMany variants of uncertain functional significance in cancer susceptibility genes lie in regulatory regions, and clarifying their association with disease risk poses significant challenges. We studied 17 germline variants (9 of which were novel) in the CDKN2A 5'UTR with independent approaches, which included mono and bicistronic reporter assays, western blot of endogenous protein, and allelic representation after polysomal profiling to investigate their impact on CDKN2A mRNA translation regulation. Two of the novel variants (c.-27del23,c.-93-91delAGG) were classified as causal mutations (score ≥3), along with the c.-21C>T,c.-34G>T and c.-56G>T, which had already been studied by a subset of assays. The novel c.-42T>A as well as the previously described c.-67G>C were classified as potential mutations (score 1 or 2). The remaining variants (c.-14C>T,c.-20A>G, c.-25C>T+c.-180G>A,c.-30G>A,c.-40C>T,c.-45G>A,c.-59C>G,c.-87T>A,c.-252A>T) were classified as neutral (score 0). In conclusion, we found evidence that nearly half of the variants found in this region had a negative impact on CDKN2A mRNA translation, supporting the hypothesis that 5'UTR can act as a cellular Internal Ribosome Entry Site (IRES) to modulate p16(INK) (4a) translation. This article is protected by copyright. All rights reserved.
| Titolo: | The CDKN2A/p16(INK) (4a) 5'UTR sequence and translational regulation: impact of novel variants predisposing to melanoma |
| Autori: | Andreotti, Virginia; Bisio, Alessandra; Bressac de Paillerets, Brigitte; Harland, Mark; Cabaret, Odile; Newton Bishop, Julia; Pastorino, Lorenza; Bruno, William; Bertorelli, Roberto; De Sanctis, Veronica; Provenzani, Alessandro; Menin, Chiara; Fronza, Gilberto; Queirolo, Paola; Spitale, Robert C; Bianchi Scarrà, Giovanna; Inga, Alberto; Ghiorzo, Paola |
| Autori Unitn: | |
| Titolo del periodico: | PIGMENT CELL & MELANOMA RESEARCH |
| Anno di pubblicazione: | 2016 |
| Numero e parte del fascicolo: | 2 |
| Codice identificativo Scopus: | 2-s2.0-84958639922 |
| Codice identificativo Pubmed: | 26581427 |
| Codice identificativo ISI: | WOS:000370645600010 |
| Digital Object Identifier (DOI): | http://dx.doi.org/10.1111/pcmr.12444 |
| Handle: | http://hdl.handle.net/11572/119322 |
| Appare nelle tipologie: | 03.1 Articolo su rivista (Journal article) |
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| File | Descrizione | Tipologia | Licenza | |
|---|---|---|---|---|
| Andreotti V et al full.pdf | Post-print referato (Refereed author’s manuscript) | Altra licenza (Other type of license) | Administrator |
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