Molecularly imprinted polymers (MIPs) were combined to MALDI-TOF-MS to evaluate a selective enrichment (SE) method for the determination of clinically relevant biomarkers from complex biological samples. The concept was proven with the myocardial injury marker Troponin I (cTnI). In a first part, MIP materials entailed for the recognition of cTnI epitopes (three peptides selected) were prepared and characterized in dimensions (0.7-2μm), dissociation constants (58-817nM), kinetics of binding (5-60min), binding capacity (ca. 1.5μg/mg polymer), imprinting factors (3>IF>5) and selectivity for the peptide epitope. Then, the MIPs, incubated with cTnI peptides and spotted on the target with the DHB matrix, were assayed for the desorption of the peptides in MALDI-TOF-MS. The measured detection limit was ca. 300femtomols. Finally, the MIP-SE MALDI-TOF-MS was tested for its ability to enrich in the cTnI peptides from a complex sample, mimic of serum (i.e. 81 peptides of digested albumin). The MIP-SE MALDI-TOF-MS successfully enriched in cTnI peptides from the complex sample proving the technique could offer a flexible platform to prepare entailed materials suitable for diagnostic purposes.

Molecularly imprinted polymers coupled to matrix assisted laser desorption ionization mass spectrometry for femtomoles detection of cardiac troponin I peptides / Cenci, L.; Anesi, A.; Busato, M.; Guella, G.; Bossi, A. M.. - In: JOURNAL OF MOLECULAR RECOGNITION. - ISSN 0952-3499. - STAMPA. - 2016:29 (1)(2016), pp. 41-50. [10.1002/jmr.2494]

Molecularly imprinted polymers coupled to matrix assisted laser desorption ionization mass spectrometry for femtomoles detection of cardiac troponin I peptides

Anesi, A.;Guella, G.;
2016-01-01

Abstract

Molecularly imprinted polymers (MIPs) were combined to MALDI-TOF-MS to evaluate a selective enrichment (SE) method for the determination of clinically relevant biomarkers from complex biological samples. The concept was proven with the myocardial injury marker Troponin I (cTnI). In a first part, MIP materials entailed for the recognition of cTnI epitopes (three peptides selected) were prepared and characterized in dimensions (0.7-2μm), dissociation constants (58-817nM), kinetics of binding (5-60min), binding capacity (ca. 1.5μg/mg polymer), imprinting factors (3>IF>5) and selectivity for the peptide epitope. Then, the MIPs, incubated with cTnI peptides and spotted on the target with the DHB matrix, were assayed for the desorption of the peptides in MALDI-TOF-MS. The measured detection limit was ca. 300femtomols. Finally, the MIP-SE MALDI-TOF-MS was tested for its ability to enrich in the cTnI peptides from a complex sample, mimic of serum (i.e. 81 peptides of digested albumin). The MIP-SE MALDI-TOF-MS successfully enriched in cTnI peptides from the complex sample proving the technique could offer a flexible platform to prepare entailed materials suitable for diagnostic purposes.
2016
29 (1)
Cenci, L.; Anesi, A.; Busato, M.; Guella, G.; Bossi, A. M.
Molecularly imprinted polymers coupled to matrix assisted laser desorption ionization mass spectrometry for femtomoles detection of cardiac troponin I peptides / Cenci, L.; Anesi, A.; Busato, M.; Guella, G.; Bossi, A. M.. - In: JOURNAL OF MOLECULAR RECOGNITION. - ISSN 0952-3499. - STAMPA. - 2016:29 (1)(2016), pp. 41-50. [10.1002/jmr.2494]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11572/117074
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