Numb-deficient satellite cells have regeneration and proliferation defects. -The first two authors equally contributed

George, R. M.; Biressi, Stefano Augusto Maria; Beres, B. J.; Rogers, E.; Mulia, A. K.; Allen, R. E.; Rawls, A.; Rando, T. a.; Wilson Rawls, J.

doi:10.1073/pnas.1311628110

The adaptor protein Numb has been implicated in the switch between cell proliferation and differentiation made by satellite cells during muscle repair. Using two genetic approaches to ablate Numb, we determined that, in its absence, muscle regeneration in response to injury was impaired. Single myofiber cultures demonstrated a lack of satellite cell proliferation in the absence of Numb, and the proliferation defect was confirmed in satellite cell cultures. Quantitative RT-PCR from Numb-deficient satellite cells demonstrated highly up-regulated expression of p21 and Myostatin, both inhibitors of myoblast proliferation. Transfection with Myostatin-specific siRNA rescued the proliferation defect of Numb-deficient satellite cells. Furthermore, overexpression of Numb in satellite cells inhibited Myostatin expression. These data indicate a unique function for Numb during the initial activation and proliferation of satellite cells in response to muscle injury.

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Titolo:	Numb-deficient satellite cells have regeneration and proliferation defects. -The first two authors equally contributed
Autori:	R. M. George; S. Biressi; B. J. Beres;E. Rogers;A. K. Mulia;R. E. Allen;A. Rawls;T. a. Rando;J. Wilson-Rawls
Autori Unitn:	R. M. George Biressi, Stefano Augusto Maria B. J. Beres E. Rogers A. K. Mulia R. E. Allen A. Rawls T. a. Rando J. Wilson Rawls mostra contributor esterni nascondi contributor esterni
Titolo del periodico:	PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Anno di pubblicazione:	2013
Codice identificativo Scopus:	2-s2.0-84887457181
Codice identificativo Pubmed:	24170859
Codice identificativo ISI:	WOS:000326830900059
Digital Object Identifier (DOI):	http://dx.doi.org/10.1073/pnas.1311628110
Handle:	http://hdl.handle.net/11572/101053
Appare nelle tipologie:	03.1 Articolo su rivista (Journal article)

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