In this work we present a bioprinting technique that exploits the electrohydrodynamic process to obtain a jet of liquid alginate beads containing cells. A printer is used to microfabricate hydrogels block by block following a bottom-up approach. Alginate beads constitute the building blocks of the microfabricated structures. The beads are placed at predefined position on a target substrate made of calcium-enriched gelatin, where they crosslink upon contact without the need of further postprocessing. The printed sample can be easily removed from the substrate at physiological temperature. Three-dimensional printing is accomplished by the deposition of multiple layers of hydrogel. We have investigated the parameters influencing the process, the compatibility of the printing procedure with cells, and their survival after printing.

An electrohydrodynamic printer for alginate hydrogels containing living cells / Gasperini, Luca; Maniglio, Devid; Motta, Antonella; Migliaresi, Claudio. - In: TISSUE ENGINEERING, PART C: METHODS. - ISSN 1937-3384. - ELETTRONICO. - 2015, 21:2(2015), pp. 123-132. [10.1089/ten.TEC.2014.0149]

An electrohydrodynamic printer for alginate hydrogels containing living cells.

Gasperini, Luca;Maniglio, Devid;Motta, Antonella;Migliaresi, Claudio
2015-01-01

Abstract

In this work we present a bioprinting technique that exploits the electrohydrodynamic process to obtain a jet of liquid alginate beads containing cells. A printer is used to microfabricate hydrogels block by block following a bottom-up approach. Alginate beads constitute the building blocks of the microfabricated structures. The beads are placed at predefined position on a target substrate made of calcium-enriched gelatin, where they crosslink upon contact without the need of further postprocessing. The printed sample can be easily removed from the substrate at physiological temperature. Three-dimensional printing is accomplished by the deposition of multiple layers of hydrogel. We have investigated the parameters influencing the process, the compatibility of the printing procedure with cells, and their survival after printing.
2015
2
Gasperini, Luca; Maniglio, Devid; Motta, Antonella; Migliaresi, Claudio
An electrohydrodynamic printer for alginate hydrogels containing living cells / Gasperini, Luca; Maniglio, Devid; Motta, Antonella; Migliaresi, Claudio. - In: TISSUE ENGINEERING, PART C: METHODS. - ISSN 1937-3384. - ELETTRONICO. - 2015, 21:2(2015), pp. 123-132. [10.1089/ten.TEC.2014.0149]
File in questo prodotto:
File Dimensione Formato  
Gasperini_2015_An electrohydrodynamic bioprinter for alginate hydrogels containing living cells.pdf

accesso aperto

Tipologia: Post-print referato (Refereed author’s manuscript)
Licenza: Tutti i diritti riservati (All rights reserved)
Dimensione 1.55 MB
Formato Adobe PDF
1.55 MB Adobe PDF Visualizza/Apri
after revision _ Gasperini.pdf

Solo gestori archivio

Tipologia: Post-print referato (Refereed author’s manuscript)
Licenza: Tutti i diritti riservati (All rights reserved)
Dimensione 6.06 MB
Formato Adobe PDF
6.06 MB Adobe PDF   Visualizza/Apri
2015 An Electrohydrodynamic Bioprinter for Alginate Hydrogels Containing Live Cells (1).pdf

Solo gestori archivio

Tipologia: Versione editoriale (Publisher’s layout)
Licenza: Tutti i diritti riservati (All rights reserved)
Dimensione 634.63 kB
Formato Adobe PDF
634.63 kB Adobe PDF   Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11572/100525
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 63
  • ???jsp.display-item.citation.isi??? 54
social impact