The present chapter describes how to investigate brain lateralization in insects with methods of optical neuroimaging. It provides two complete protocols, one for in vivo imaging to obtain information on functional lateralization, and one for histochemical techniques to study morphological asymmetries. Both sections start with the animal preparation, illustrate the different possibilities for brain tissue labeling, and review the available imaging techniques, concentrating on widefield fluorescence microscopy, confocal, and two-photon laser scanning microscopy. After some remarks on the main methods for data analysis, studies on functional and morphological lateralization in insects are reviewed.
Imaging Techniques in Insects / Paoli, Marco; Andrione, Mara; Haase, Albrecht. - 217:(2025), pp. 591-638. [10.1007/978-1-0716-4240-5_17]
Imaging Techniques in Insects
Paoli, Marco
Primo
;Andrione, MaraSecondo
;Haase, Albrecht
Ultimo
2025-01-01
Abstract
The present chapter describes how to investigate brain lateralization in insects with methods of optical neuroimaging. It provides two complete protocols, one for in vivo imaging to obtain information on functional lateralization, and one for histochemical techniques to study morphological asymmetries. Both sections start with the animal preparation, illustrate the different possibilities for brain tissue labeling, and review the available imaging techniques, concentrating on widefield fluorescence microscopy, confocal, and two-photon laser scanning microscopy. After some remarks on the main methods for data analysis, studies on functional and morphological lateralization in insects are reviewed.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione
