Engineered genetic circuits with tailored functions that mimic how cells process information in changing environments (e.g. cell fate decision, chemotaxis, immune response) have great applications in biomedicine and synthetic biology. Although there is a lot of progress toward the design of gene circuits yielding desired steady states (e.g. logic-based networks), building synthetic circuits for dynamic signal processing (e.g. filters, frequency modulation, and controllers) is still challenging. Here, we provide a model-based approach to build gene networks that can operate as band-pass filters by taking advantage of molecular sequestration. By suitably approximating the dynamics of molecular sequestration, we analyze an Incoherent Feed-Forward Loop (IFFL) and a Negative Feedback (NF) circuit and illustrate how they can achieve band-pass filter behavior. Computational analysis shows that a circuit that incorporates both IFFL and NF motifs improves the filter performance. Our approach facilitates the design of sequestration-based filters, and may support the synthesis of molecular controllers with desired specifications.

Building molecular band-pass filters via molecular sequestration / Zhang, Yichi; Samaniego, Christian Cuba; Carleton, Katelyn; Qian, Yili; Giordano, Giulia; Franco, Elisa. - (2022), pp. 3890-3895. (Intervento presentato al convegno CDC 2022 tenutosi a Cancun, Mexico nel 6th-9th December 2022) [10.1109/CDC51059.2022.9993401].

Building molecular band-pass filters via molecular sequestration

Giordano, Giulia
Penultimo
;
2022-01-01

Abstract

Engineered genetic circuits with tailored functions that mimic how cells process information in changing environments (e.g. cell fate decision, chemotaxis, immune response) have great applications in biomedicine and synthetic biology. Although there is a lot of progress toward the design of gene circuits yielding desired steady states (e.g. logic-based networks), building synthetic circuits for dynamic signal processing (e.g. filters, frequency modulation, and controllers) is still challenging. Here, we provide a model-based approach to build gene networks that can operate as band-pass filters by taking advantage of molecular sequestration. By suitably approximating the dynamics of molecular sequestration, we analyze an Incoherent Feed-Forward Loop (IFFL) and a Negative Feedback (NF) circuit and illustrate how they can achieve band-pass filter behavior. Computational analysis shows that a circuit that incorporates both IFFL and NF motifs improves the filter performance. Our approach facilitates the design of sequestration-based filters, and may support the synthesis of molecular controllers with desired specifications.
2022
2022 IEEE 61st Conference on Decision and Control (CDC)
Amsterdam
Institute of Electrical and Electronics Engineers Inc.
978-1-6654-6761-2
Zhang, Yichi; Samaniego, Christian Cuba; Carleton, Katelyn; Qian, Yili; Giordano, Giulia; Franco, Elisa
Building molecular band-pass filters via molecular sequestration / Zhang, Yichi; Samaniego, Christian Cuba; Carleton, Katelyn; Qian, Yili; Giordano, Giulia; Franco, Elisa. - (2022), pp. 3890-3895. (Intervento presentato al convegno CDC 2022 tenutosi a Cancun, Mexico nel 6th-9th December 2022) [10.1109/CDC51059.2022.9993401].
File in questo prodotto:
File Dimensione Formato  
CP041_2022_CDC_biomolecular.pdf

accesso aperto

Tipologia: Post-print referato (Refereed author’s manuscript)
Licenza: Tutti i diritti riservati (All rights reserved)
Dimensione 917.29 kB
Formato Adobe PDF
917.29 kB Adobe PDF Visualizza/Apri
Building_molecular_band-pass_filters_via_molecular_sequestration.pdf

Solo gestori archivio

Tipologia: Versione editoriale (Publisher’s layout)
Licenza: Tutti i diritti riservati (All rights reserved)
Dimensione 1.64 MB
Formato Adobe PDF
1.64 MB Adobe PDF   Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11572/409572
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 0
  • ???jsp.display-item.citation.isi??? 0
social impact