During the last decade three-dimensional (3D) cultures of pluripotent stem cells have been intensively used to understand morphogenesis and molecular signaling important for the embryonic development of many tissues. In addition, pluripotent stem cells have been shown to be a valid tool for the in vitro model- ing of several congenital or chronic human diseases, opening new possibilities to study their physiopathol- ogy without using animal models. Even more interestingly, 3D culture has proved to be a powerful and versatile tool to successfully generate functional tissues ex vivo. Using similar approaches, we here describe a protocol for the generation of functional thyroid tissue using mouse embryonic stem cells and give all the details and references for its characterization and analysis both in vitro and in vivo. This model is a valid approach to study the expression and the function of genes involved in the correct morphogenesis of thyroid gland, to elucidate the mechanisms of production and secretion of thyroid hormones and to test anti-thyroid drugs.

Generation of Functional Thyroid Tissue Using 3D-Based Culture of Embryonic Stem Cells / Antonica, Francesco; Kasprzyk, Dominika Figini; Schiavo, Andrea Alex; Romitti, Mírian; Costagliola, Sabine. - 1597:(2017), pp. 85-95. [10.1007/978-1-4939-6949-4_7]

Generation of Functional Thyroid Tissue Using 3D-Based Culture of Embryonic Stem Cells

Antonica, Francesco;
2017-01-01

Abstract

During the last decade three-dimensional (3D) cultures of pluripotent stem cells have been intensively used to understand morphogenesis and molecular signaling important for the embryonic development of many tissues. In addition, pluripotent stem cells have been shown to be a valid tool for the in vitro model- ing of several congenital or chronic human diseases, opening new possibilities to study their physiopathol- ogy without using animal models. Even more interestingly, 3D culture has proved to be a powerful and versatile tool to successfully generate functional tissues ex vivo. Using similar approaches, we here describe a protocol for the generation of functional thyroid tissue using mouse embryonic stem cells and give all the details and references for its characterization and analysis both in vitro and in vivo. This model is a valid approach to study the expression and the function of genes involved in the correct morphogenesis of thyroid gland, to elucidate the mechanisms of production and secretion of thyroid hormones and to test anti-thyroid drugs.
2017
Organ Regeneration: 3D Stem Cell Culture & Manipulation
999 RIVERVIEW DR, STE 208, TOTOWA, NJ 07512-1165 USA
HUMANA PRESS INC
978-1-4939-6947-0
978-1-4939-6949-4
Antonica, Francesco; Kasprzyk, Dominika Figini; Schiavo, Andrea Alex; Romitti, Mírian; Costagliola, Sabine
Generation of Functional Thyroid Tissue Using 3D-Based Culture of Embryonic Stem Cells / Antonica, Francesco; Kasprzyk, Dominika Figini; Schiavo, Andrea Alex; Romitti, Mírian; Costagliola, Sabine. - 1597:(2017), pp. 85-95. [10.1007/978-1-4939-6949-4_7]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11572/352852
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