Advancements in fluorescent microscopy techniques now permit investigation of HIV-1 biology exploiting tools alternative to conventional molecular biology. Here we describe a novel, fluorescence-based method to visualize HIV-1 viral particles within intact nuclei of infected cells. This method allows investigating the localization of pre-integration complexes within the nuclear compartment with respect to the nuclear envelope and the chromatin territories.
Imaging HIV-1 Nuclear Pre-integration Complexes / Cereseto, Anna; M., Giacca. - 1087:(2013), pp. 47-54. [10.1007/978-1-62703-670-2_5]
Imaging HIV-1 Nuclear Pre-integration Complexes.
Cereseto, Anna;
2013-01-01
Abstract
Advancements in fluorescent microscopy techniques now permit investigation of HIV-1 biology exploiting tools alternative to conventional molecular biology. Here we describe a novel, fluorescence-based method to visualize HIV-1 viral particles within intact nuclei of infected cells. This method allows investigating the localization of pre-integration complexes within the nuclear compartment with respect to the nuclear envelope and the chromatin territories.File in questo prodotto:
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