In seismic countries, the managing authorities of road networks rely on risk assessment of bridges to decide retrofit intervention priorities on a regional scale. It is a current practice to assess seismic vulnerability in the form of fragility curves. The most extensively used tool currently available for the evaluation of vulnerability curves of large-scale systems is the Hazus methodology, which provides fragility curves for whole classes of structures, but shows deficiencies when applied to a community–specific database, where different typologies are represented. Given the large number of bridges in a regional scale road network, fragility curves have to be obtained by means of simplified and reliable numerical methods, time-history analyses being to date still very expensive in computational terms when applied in a probabilistic framework. In the last decade displacement-based (DB) methods have become increasingly established simplified assessment procedures. Present work reports on an extensive risk assessment study carried out on Northern-Italy’s Region of Veneto road system; the analysis focuses on one of the most typical bridge types of the network, multi-span RC bridges. Analytical fragility curves obtained with displacement-based approaches are proved to be sufficiently accurate. The curves obtained can be used as a reference for Italian and European RC multi-span concrete bridges.