microRNA capture affinity technology (miR-CATCH) uses affinity capture biotinylated antisense oligonucleotides to co-purify a target transcript together with all its endogenously bound miRNAs. The miR-CATCH assay is performed to investigate miRNAs bound to a specific mRNA. This method allows to have a total vision of miRNAs bound not only to the 3′UTR but also to the 5′UTR and Coding Region of target messenger RNAs (mRNAs).

Identification of miRNAs Bound to an RNA of Interest by MicroRNA Capture Affinity Technology (miR-CATCH) / Zeni, A.; Grasso, M.; Denti, M. A.. - ELETTRONICO. - 2404:(2022), pp. 207-218. [10.1007/978-1-0716-1851-6_11]

Identification of miRNAs Bound to an RNA of Interest by MicroRNA Capture Affinity Technology (miR-CATCH)

Grasso M.;Denti M. A.
2022-01-01

Abstract

microRNA capture affinity technology (miR-CATCH) uses affinity capture biotinylated antisense oligonucleotides to co-purify a target transcript together with all its endogenously bound miRNAs. The miR-CATCH assay is performed to investigate miRNAs bound to a specific mRNA. This method allows to have a total vision of miRNAs bound not only to the 3′UTR but also to the 5′UTR and Coding Region of target messenger RNAs (mRNAs).
2022
Methods in Molecular Biology
Berlin, New York
Humana Press Inc.
978-1-0716-1850-9
978-1-0716-1851-6
Zeni, A.; Grasso, M.; Denti, M. A.
Identification of miRNAs Bound to an RNA of Interest by MicroRNA Capture Affinity Technology (miR-CATCH) / Zeni, A.; Grasso, M.; Denti, M. A.. - ELETTRONICO. - 2404:(2022), pp. 207-218. [10.1007/978-1-0716-1851-6_11]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11572/333604
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