Exploiting Intestinal Organoids and Foodomics Strategies for Studying the Role of Diet and Host Responses

Complex problems need complex solutions, particularly when tackling the study of diet and host interactions in homeostasis and disease, such as colorectal cancer (CRC). A myriad of factors crosslinking diet, gut microbiome, and genes must be taken into account in order to achieve solid conclusions. Recently, intestinal organoids have significantly provided a better model system in this endeavor. These emerging models mimic in vivo conditions much better than traditional cultures. In this chapter, we discuss how these emerging models may bring a completely new approach to the study gut epithelium responses to diet-related metabolites in homeostasis and CRC disease, how these 3D cultures are established, and lastly, the use of state-of-the-art of mass spectrometry and data processing techniques to obtain useful insights.