Although Deoxyribonucleic acid (DNA) is considered substantially stable in aqueous solution, slow hydrolysis can damage its double-helix structure and cause denaturation when it is stored for several months. Therefore, the design of aqueous solvents that are able to stabilize and maintain DNA conformation is a challenging issue. Ionic liquids (ILs) appear as ideal water co-solvents for DNA biotechnology due to their unique properties. We have investigated the thermal stability of DNA in 1-butyl-3-methylimidazolium aqueous solutions by synchrotron-based UV Resonance Raman (UVRR) spectroscopy with the aim to clarify the role played by concentration of IL in stabilizing the DNA natural conformation. The synchrotron-based UV source for UVRR measurements allows us to enhance specific vibrational signals associated to nitrogenous bases of DNA, through an appropriate tuning of the excitation wavelength. Such approach permits to probe the rearrangements in the local environment around specific nucleotides as a function of thermal conditions

Conformational stability of DNA in hydrated ionic liquid by synchrotron-based UV resonance raman / Bottari, Cettina; Mancini, Ines; Mele, Andrea; Gessini, Alessandro; Masciovecchio, Claudio; Rossi, Barbara. - ELETTRONICO. - Vol. 11086:(2019), pp. 24-31. (Intervento presentato al convegno UV and Higher Energy Photonics: From Materials to Applications 2019 tenutosi a San Diego, CA nel 9 September 2019) [10.1117/12.2529077].

Conformational stability of DNA in hydrated ionic liquid by synchrotron-based UV resonance raman

Mancini, Ines;Rossi, Barbara
2019-01-01

Abstract

Although Deoxyribonucleic acid (DNA) is considered substantially stable in aqueous solution, slow hydrolysis can damage its double-helix structure and cause denaturation when it is stored for several months. Therefore, the design of aqueous solvents that are able to stabilize and maintain DNA conformation is a challenging issue. Ionic liquids (ILs) appear as ideal water co-solvents for DNA biotechnology due to their unique properties. We have investigated the thermal stability of DNA in 1-butyl-3-methylimidazolium aqueous solutions by synchrotron-based UV Resonance Raman (UVRR) spectroscopy with the aim to clarify the role played by concentration of IL in stabilizing the DNA natural conformation. The synchrotron-based UV source for UVRR measurements allows us to enhance specific vibrational signals associated to nitrogenous bases of DNA, through an appropriate tuning of the excitation wavelength. Such approach permits to probe the rearrangements in the local environment around specific nucleotides as a function of thermal conditions
2019
PROCEEDINGS OF SPIE; Event: SPIE Nanoscience + Engineering, 2019, San Diego, California, United States
Bottari, C.; Mancini, I.; Mele, A.; Gessinia, A.; Masciovecchio, C.; Rossi, B.
San Diego, CA
spiedidigitalibrary
9781510628656
9781510628663
Bottari, Cettina; Mancini, Ines; Mele, Andrea; Gessini, Alessandro; Masciovecchio, Claudio; Rossi, Barbara
Conformational stability of DNA in hydrated ionic liquid by synchrotron-based UV resonance raman / Bottari, Cettina; Mancini, Ines; Mele, Andrea; Gessini, Alessandro; Masciovecchio, Claudio; Rossi, Barbara. - ELETTRONICO. - Vol. 11086:(2019), pp. 24-31. (Intervento presentato al convegno UV and Higher Energy Photonics: From Materials to Applications 2019 tenutosi a San Diego, CA nel 9 September 2019) [10.1117/12.2529077].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11572/242811
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