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EnglishVarious species of non-coding RNAs (ncRNAs) are enriched in subcellular compartments but the mechanisms orchestrating their delocalization and their local functions remain largely unknown. We investigated both aspects using the elongating retinal ganglion cell axon and its tip, the growth cone, as models. We reveal that specific endogenous precursor microRNAs (pre-miRNAs) are actively trafficked, anchored to CD63-positive vesicles, to distal axons along microtubules. Upon exposure to the chemotropic cue Sema3A, pre-miRNAs are processed specifically within axons into newly synthesized mature miRNAs, which, in turn, silence the basal translation of TUBB3 but not of APP. At the organismal level, these mature miRNAs are required for growth cone steering and a fully functional visual system. Overall, our results uncover a novel mode of ncRNA transport from one cytosolic compartment to another within polarized cells. They also reveal that newly synthesized miRNAs are critical components of a ncRNA-based signaling pathway that transduces environmental signals into the structural remodelling of subcellular compartments.
Precursor miRNAs are trafficked along axons associated with vesicles and locally processed to regulate growth cone steering / Corradi, Eloina; Gavoci, Antoneta; Strohbuecker, Stephanie Elisabeth; Roccuzzo, Michela; Dalla Costa, Irene; Iyer, Archana; Bridi, Simone; Santoz Rodriguez, Gabriela; Abreu-Goodger, Cei; Baudet, Marie-Laure. - ELETTRONICO. - (2018). [10.1101/470393]
| Titolo: | Precursor miRNAs are trafficked along axons associated with vesicles and locally processed to regulate growth cone steering | |
| Autori: | Corradi, Eloina; Gavoci, Antoneta; Strohbuecker, Stephanie Elisabeth; Roccuzzo, Michela; Dalla Costa, Irene; Iyer, Archana; Bridi, Simone; Santoz Rodriguez, Gabriela; Abreu-Goodger, Cei; Baudet, Marie-Laure | |
| Autori Unitn: | ||
| Luogo di edizione: | Cold Spring Harbor | |
| Casa editrice: | Cold Spring Harbor Laboratory | |
| Anno di pubblicazione: | 2018 | |
| Handle: | http://hdl.handle.net/11572/228472 | |
| Citazione: | Precursor miRNAs are trafficked along axons associated with vesicles and locally processed to regulate growth cone steering / Corradi, Eloina; Gavoci, Antoneta; Strohbuecker, Stephanie Elisabeth; Roccuzzo, Michela; Dalla Costa, Irene; Iyer, Archana; Bridi, Simone; Santoz Rodriguez, Gabriela; Abreu-Goodger, Cei; Baudet, Marie-Laure. - ELETTRONICO. - (2018). [10.1101/470393] | |
| Appare nelle tipologie: | 07.2 Altre pubblicazioni (Other types of publications) |
File in questo prodotto:
| File | Descrizione | Tipologia | Licenza | |
|---|---|---|---|---|
| 470393.full.pdf | articolo principale | Pre-print non referato (Non-refereed preprint) |  | Open Access Visualizza/Apri |
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