Extracellular vesicles (EVs) are heterogeneous membranous particles secreted from the cells. They are abundant in biological fluids and transport a variety of biomolecules, such as proteins, lipids, and nucleic acids, reflecting the cell of origin. We applied a new method, developed for an efficient, fast and reliable isolation of EVs, in combination with amplified luminescent proximity homogeneous assay (alpha) technology in order to detect EV-surface antigens in the ultrasensitive range. We have measured PSA-positive EV sub-populations derived from prostate cancer cell lines with the possibility, for the first time, to use them as the readout in a high-throughput screening platform.

Tumor-derived extracellular vesicles as a readout for high-throughput screenings / D'Agostino, Vito Giuseppe; Adami, Valentina; Gatto, Pamela; Sidarovich, Viktoryia; Pancher, Michael. - (2018). ((Intervento presentato al convegno BioFacility Day tenutosi a Trento nel 15/02/2018.

Tumor-derived extracellular vesicles as a readout for high-throughput screenings

Vito Giuseppe D'Agostino;Valentina Adami;Pamela Gatto;Viktoryia Sidarovich;Michael Pancher
2018

Abstract

Extracellular vesicles (EVs) are heterogeneous membranous particles secreted from the cells. They are abundant in biological fluids and transport a variety of biomolecules, such as proteins, lipids, and nucleic acids, reflecting the cell of origin. We applied a new method, developed for an efficient, fast and reliable isolation of EVs, in combination with amplified luminescent proximity homogeneous assay (alpha) technology in order to detect EV-surface antigens in the ultrasensitive range. We have measured PSA-positive EV sub-populations derived from prostate cancer cell lines with the possibility, for the first time, to use them as the readout in a high-throughput screening platform.
BioFacility Day
Tumor-derived extracellular vesicles as a readout for high-throughput screenings / D'Agostino, Vito Giuseppe; Adami, Valentina; Gatto, Pamela; Sidarovich, Viktoryia; Pancher, Michael. - (2018). ((Intervento presentato al convegno BioFacility Day tenutosi a Trento nel 15/02/2018.
D'Agostino, Vito Giuseppe; Adami, Valentina; Gatto, Pamela; Sidarovich, Viktoryia; Pancher, Michael
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11572/197854
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