2026-09-19T05:24:36Zhttps://iris.unitn.it/oai/requestoai:iris.unitn.it:11572/3685122026-04-03T00:50:13Zcom_11572_237821com_11572_101871col_11572_237822ou_ou00279
Imaging Chloride Homeostasis in Neurons
Arosio, Daniele
Arosio, Daniele
Dalla Serra, Mauro
Settore BIO/11 - Biologia Molecolare
Settore FIS/07 - Fisica Applicata(Beni Culturali, Ambientali, Biol.e Medicin)
Settore BIO/10 - Biochimica
Settore BIO/13 - Biologia Applicata
Intracellular chloride and pH are fundamental regulators of neuronal excitability and they are often co-modulated during excitation-inhibition activity. The study of their homeostasis requires simultaneous measurements in vivo in multiple neurons. Combining random mutagenesis screening, protein engineering and two-photon-imaging this thesis work led to the discovery of new chloride-sensitive GFP mutants and to the establishment of ratiometric imaging procedures for the quantitative combined imaging of intraneuronal pH and chloride. These achievements have been demonstrated in vivo in the mouse cortex, in real-time monitoring the dynamic changes of ions concentrations during epileptic-like discharges, and in glioblastoma primary cells, measuring osmotic swelling responses to various drugs treatment.
2017
info:eu-repo/semantics/doctoralThesis
https://hdl.handle.net/11572/368512
http://dx.doi.org/10.15168/11572_368512
10.15168/11572_368512
eng
firstpage:1
lastpage:190
numberofpages:190
info:eu-repo/semantics/openAccess
Università degli studi di Trento
place:TRENTO
license:Tutti i diritti riservati (All rights reserved)
license:Tutti i diritti riservati (All rights reserved)
license uri:iris.PRI01