Advancements in fluorescent microscopy techniques now permit investigation of HIV-1 biology exploiting tools alternative to conventional molecular biology. Here we describe a novel, fluorescence-based method to visualize HIV-1 viral particles within intact nuclei of infected cells. This method allows investigating the localization of pre-integration complexes within the nuclear compartment with respect to the nuclear envelope and the chromatin territories.

Imaging HIV-1 Nuclear Pre-integration Complexes / Cereseto, Anna; M., Giacca. - 1087:(2013), pp. 47-54. [10.1007/978-1-62703-670-2_5]

Imaging HIV-1 Nuclear Pre-integration Complexes.

Cereseto, Anna;
2013-01-01

Abstract

Advancements in fluorescent microscopy techniques now permit investigation of HIV-1 biology exploiting tools alternative to conventional molecular biology. Here we describe a novel, fluorescence-based method to visualize HIV-1 viral particles within intact nuclei of infected cells. This method allows investigating the localization of pre-integration complexes within the nuclear compartment with respect to the nuclear envelope and the chromatin territories.
2013
Human Retroviruses
USA
Humana Press Springer Science+Businnes Media, LLC
978-1-62703-669-6
Cereseto, Anna; M., Giacca
Imaging HIV-1 Nuclear Pre-integration Complexes / Cereseto, Anna; M., Giacca. - 1087:(2013), pp. 47-54. [10.1007/978-1-62703-670-2_5]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11572/34314
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