Photosynthetic Changes That Occur During Aging of Cypress (Cupressus sempervirens L.) Needles

2-years-old cypress needles (A2) were physiologically most active with regard to net photosynthetic (P N) and electron transport rates. Variable to maximum fluorescence (Fv/Fm) ratios of dark-adapted needles were higher in A2 needles than in current year (A1) or senescent (A4) needles. Lower Fv/Fm values in these stages seemed to be caused not by photoinhibition but by a low photochemical capacity as suggested from the chlorophyll (Chl) a/b ratios. In isolated thylakoids, lower rates of whole chain and photosystem 2 (PS2) activities were observed in A4 needles, while higher rates were observed in A2 needles. A similar trend was noticed for contents of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBPC) and total soluble proteins. The artificial exogenous electron donor Mn 2+ failed to restore the loss of PS2 activity in 3-year-old (A3) and A4 needles, while diphenylcarbazide and NH2OH significantly restored the loss of PS2 activity. The marked loss of PS2 activity in A4 needles was primarily the result of the loss of 33, 28-25, 23, and 17 kDa polypeptides. A marked loss of RuBPC activity in A4 needles is mainly due to the loss of 15 (SSU) and 55 (LSU) kDa polypeptides. © 2006 Institute of Experimental Botany, ASCR.