The bromodomain-containing protein BAZ2A is a validated target in prostate cancer research, whereas the function of its paralogue BAZ2B is still undefined. The bromodomains of BAZ2A and BAZ2B have a similar binding site for their natural ligand, the acetylated lysine side chain. Here, we present an analysis of the binding modes of eight compounds belonging to three distinct chemical classes. For all compounds, the moiety mimicking the natural ligand engages in essentially identical interactions in the BAZ2A and BAZ2B bromodomains. In contrast, the rest of the molecule is partially solvent-exposed and adopts different orientations with different interactions in the two bromodomains. Some of these differences could be exploited for designing inhibitors with selectivity within the BAZ2 bromodomain subfamily.

Structural Analysis of Small-Molecule Binding to the BAZ2A and BAZ2B Bromodomains / Dalle Vedove, A.; Spiliotopoulos, D.; D'Agostino, V. G.; Marchand, J. -R.; Unzue, A.; Nevado, C.; Lolli, G.; Caflisch, A.. - In: CHEMMEDCHEM. - ISSN 1860-7179. - 2018, 13:14(2018), pp. 1479-1487. [10.1002/cmdc.201800234]

Structural Analysis of Small-Molecule Binding to the BAZ2A and BAZ2B Bromodomains

Dalle Vedove A.;D'Agostino V. G.;Lolli G.;
2018-01-01

Abstract

The bromodomain-containing protein BAZ2A is a validated target in prostate cancer research, whereas the function of its paralogue BAZ2B is still undefined. The bromodomains of BAZ2A and BAZ2B have a similar binding site for their natural ligand, the acetylated lysine side chain. Here, we present an analysis of the binding modes of eight compounds belonging to three distinct chemical classes. For all compounds, the moiety mimicking the natural ligand engages in essentially identical interactions in the BAZ2A and BAZ2B bromodomains. In contrast, the rest of the molecule is partially solvent-exposed and adopts different orientations with different interactions in the two bromodomains. Some of these differences could be exploited for designing inhibitors with selectivity within the BAZ2 bromodomain subfamily.
2018
14
Dalle Vedove, A.; Spiliotopoulos, D.; D'Agostino, V. G.; Marchand, J. -R.; Unzue, A.; Nevado, C.; Lolli, G.; Caflisch, A.
Structural Analysis of Small-Molecule Binding to the BAZ2A and BAZ2B Bromodomains / Dalle Vedove, A.; Spiliotopoulos, D.; D'Agostino, V. G.; Marchand, J. -R.; Unzue, A.; Nevado, C.; Lolli, G.; Caflisch, A.. - In: CHEMMEDCHEM. - ISSN 1860-7179. - 2018, 13:14(2018), pp. 1479-1487. [10.1002/cmdc.201800234]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11572/282465
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