RNA G-quadruplexes (RG4s) are four-stranded structures known to control mRNA translation of cancer relevant genes. RG4 formation is pervasive in vitro but not in cellulo, indicating the existence of poorly characterized molecular machinery that remodels RG4s and maintains them unfolded. Here, we performed a quantitative proteomic screen to identify cytosolic proteins that interact with a canonical RG4 in its folded and unfolded conformation. Our results identified hnRNP H/F as important components of the cytoplasmic machinery modulating the structural integrity of RG4s, revealed their function in RG4-mediated translation and uncovered the underlying molecular mechanism impacting the cellular stress response linked to the outcome of glioblastoma.
hnRNP H/F drive RNA G-quadruplex-mediated translation linked to genomic instability and therapy resistance in glioblastoma / Herviou, P.; Le Bras, M.; Dumas, L.; Hieblot, C.; Gilhodes, J.; Cioci, G.; Hugnot, J. -P.; Ameadan, A.; Guillonneau, F.; Dassi, E.; Cammas, A.; Millevoi, S.. - In: NATURE COMMUNICATIONS. - ISSN 2041-1723. - 11:1(2020), p. 2661.
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Titolo: | hnRNP H/F drive RNA G-quadruplex-mediated translation linked to genomic instability and therapy resistance in glioblastoma |
Autori: | Herviou, P.; Le Bras, M.; Dumas, L.; Hieblot, C.; Gilhodes, J.; Cioci, G.; Hugnot, J. -P.; Ameadan, A.; Guillonneau, F.; Dassi, E.; Cammas, A.; Millevoi, S. |
Autori Unitn: | |
Titolo del periodico: | NATURE COMMUNICATIONS |
Anno di pubblicazione: | 2020 |
Numero e parte del fascicolo: | 1 |
Codice identificativo Scopus: | 2-s2.0-85085472641 |
Codice identificativo Pubmed: | 32461552 |
Codice identificativo ISI: | WOS:000538030400017 |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1038/s41467-020-16168-x |
Handle: | http://hdl.handle.net/11572/268830 |
Citazione: | hnRNP H/F drive RNA G-quadruplex-mediated translation linked to genomic instability and therapy resistance in glioblastoma / Herviou, P.; Le Bras, M.; Dumas, L.; Hieblot, C.; Gilhodes, J.; Cioci, G.; Hugnot, J. -P.; Ameadan, A.; Guillonneau, F.; Dassi, E.; Cammas, A.; Millevoi, S.. - In: NATURE COMMUNICATIONS. - ISSN 2041-1723. - 11:1(2020), p. 2661. |
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