The application of D-ribose in chemistry and pharmacy lately has received much attention. In order to develop an efficient D-ribose production process, a screening strategy was established to isolate naturally occurring D-ribose secreting microorganisms. Of the more than two thousand strains isolated, only four excreted D-ribose in a range from 0.04 to 0.36 g/l, starting from 30 g/l of D-glucose. One strain, identified as Candida pelliculosa, quantitatively co-produced D-arabitol (up to 7 g/l). This strain was subsequently submitted to UV-mutagenesis and a selective nystatin-based enrichment, leading to the isolation of a D-arabitol non-secreting mutant (C. pelliculosa PDW 43 ARA-), which synthesized 5 g/l of D-ribose from 30 g/l of D-glucose.
Screening and mutational improvement of a D-ribose secreting Candida pelliculosa strain / De Wulf, P.; Soetaert, W.; Schwengers, D.; Vandamme, E. J.. - In: JOURNAL OF FERMENTATION AND BIOENGINEERING. - ISSN 0922-338X. - ELETTRONICO. - 1996, vol. 82:1(1996), pp. 1-7. [10.1016/0922-338X(96)89446-3]
Screening and mutational improvement of a D-ribose secreting Candida pelliculosa strain
De Wulf P.;
1996-01-01
Abstract
The application of D-ribose in chemistry and pharmacy lately has received much attention. In order to develop an efficient D-ribose production process, a screening strategy was established to isolate naturally occurring D-ribose secreting microorganisms. Of the more than two thousand strains isolated, only four excreted D-ribose in a range from 0.04 to 0.36 g/l, starting from 30 g/l of D-glucose. One strain, identified as Candida pelliculosa, quantitatively co-produced D-arabitol (up to 7 g/l). This strain was subsequently submitted to UV-mutagenesis and a selective nystatin-based enrichment, leading to the isolation of a D-arabitol non-secreting mutant (C. pelliculosa PDW 43 ARA-), which synthesized 5 g/l of D-ribose from 30 g/l of D-glucose.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione
