Soft molecularly imprinted nanogels (nanoMIPs), selective for human transferrin (HTR), were prepared via a template assisted synthesis. Owing to their soft matter, the nanoMIPs were observed to deform at binding to HTR: while no relevant changes were observed in the hydrodynamic sizes of HTR-free compared to HTR-loaded nanoMIPs, the HTR binding resulted in a significant increment of the nanoMIP stiffness, with the mean Young's modulus measured by AFM passing from 17 ± 6 kPa to 56 ± 18 kPa. When coupled to a plastic optical fibre (POF) plasmonic platform, the analyte-induced nanoMIP-deformations amplified the resonance shift, enabling to attain ultra-low sensitivities (LOD = 1.2 fM; linear dynamic range of concentrations from 1.2 fM to 1.8 pM). Therefore, soft molecularly imprinted nanogels that obey to analyte-induced deformation stand as a novel class of sensitivity-gain structures for plasmonic sensing.

Deformable molecularly imprinted nanogels permit sensitivity-gain in plasmonic sensing / Cennamo, N.; Maniglio, D.; Tatti, R.; Zeni, L.; Bossi, A. M.. - In: BIOSENSORS & BIOELECTRONICS. - ISSN 0956-5663. - 156:(2020), p. 112126. [10.1016/j.bios.2020.112126]

Deformable molecularly imprinted nanogels permit sensitivity-gain in plasmonic sensing

Maniglio D.;Tatti R.;
2020-01-01

Abstract

Soft molecularly imprinted nanogels (nanoMIPs), selective for human transferrin (HTR), were prepared via a template assisted synthesis. Owing to their soft matter, the nanoMIPs were observed to deform at binding to HTR: while no relevant changes were observed in the hydrodynamic sizes of HTR-free compared to HTR-loaded nanoMIPs, the HTR binding resulted in a significant increment of the nanoMIP stiffness, with the mean Young's modulus measured by AFM passing from 17 ± 6 kPa to 56 ± 18 kPa. When coupled to a plastic optical fibre (POF) plasmonic platform, the analyte-induced nanoMIP-deformations amplified the resonance shift, enabling to attain ultra-low sensitivities (LOD = 1.2 fM; linear dynamic range of concentrations from 1.2 fM to 1.8 pM). Therefore, soft molecularly imprinted nanogels that obey to analyte-induced deformation stand as a novel class of sensitivity-gain structures for plasmonic sensing.
2020
Cennamo, N.; Maniglio, D.; Tatti, R.; Zeni, L.; Bossi, A. M.
Deformable molecularly imprinted nanogels permit sensitivity-gain in plasmonic sensing / Cennamo, N.; Maniglio, D.; Tatti, R.; Zeni, L.; Bossi, A. M.. - In: BIOSENSORS & BIOELECTRONICS. - ISSN 0956-5663. - 156:(2020), p. 112126. [10.1016/j.bios.2020.112126]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11572/255369
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